Identifikasi Fraksi Bioaktif Bawang Merah Palu (Allium x wakegi Araki.) sebagai Antibakteri terhadap Salmonella typhi dan MRSA melalui KLT-Bioautografi

Sitti Sari; Almira Rayyah Shadriah Fahru

doi:10.32529/jbb.v4i01.3967

Authors

Sitti Sari Muhammadiyah Luwuk University
Almira Rayyah Shadriah Fahru Universitas Muhammadiyah Luwuk

DOI:

https://doi.org/10.32529/jbb.v4i01.3967

Keywords:

Antibiotic resistance, Phytochemical fractionation, Secondary metabolites

Abstract

Bacterial resistance to antibiotics encourages the development of alternative antibacterial agents based on natural ingredients. Shallots (Allium x wakegi Araki.) Palu cultivars are known to contain secondary metabolites such as flavonoids, alkaloids, and steroids that have the potential as antibacterials. This study aims to evaluate the antibacterial activity of the ethanol extract fraction of Palu shallot bulbs against Salmonella typhi and Methicillin-Resistant Staphylococcus aureus (MRSA), and to identify the bioactive compounds contained therein. The thick extract was obtained through 96% ethanol maceration and then fractionated using Vacuum Liquid Chromatography (VLC). Identification of compounds was carried out using Thin Layer Chromatography (TLC) and specific detection reagents (Dragendorff, Liebermann-Burchard, AlCl?, FeCl?). The antibacterial activity of each fraction was tested using the direct bioautography method. The results of the study are expected to reveal the active fractions and the dominant bioactive compounds as natural antibacterial candidates. These findings are expected to support the utilization of local biological resources as an alternative in the development of plant-based antimicrobial agents, especially from the Palu red onion cultivar which has high adaptive and bioactive potential.

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